The expression level of IFI۶ gene in Breast cancer patients regulated by mir-۴۲۹۸

Introduction Each year, ۲.۳ million new breast cancer patients are diagnosed, and women constitute a large portion of CBC cancer cases. In this case study, we focus on the interferon alpha/beta signaling pathway, which plays a crucial role in CBC invasion and proliferation of cancerous cells. We analyze the expression level of a special gene regulated by the biological effects of miRNA and lncRNA using bioinformatics analysis. Materials and methods In the first step, a suitable GSE (GSE۱۳۴۳۵۹) is downloaded from the GEO database and analyzed by GEO۲R. The preferred gene is selected with the most significant high expression based on checking by GEPIA۲, and ENCORI are used to verify logFC and P.Val. The signaling pathway that interfered in CBC promotion selected by Enrichr is confirmed by the REACTOM database. Moreover, direct correlation of targeted miRNA with the mRNA validated based on the ENCORI database. lncRNA selected in the lncRResearch database as related lncRNA with mentioned gene. The protein-protein interaction of the selected gene was analyzed by the STRING database. After analyzing SNPs related to the said gene through the SIFT, miRNASNP, and dbSNP databases, a SNP in the ۳’UTR region named rs۹۲۴۹۵۹۱۷۹ was selected, and a SNP in the coding sequence was selected, and their effects on the target gene were analyzed with the Hope database. Interaction between the target gene and its associated miRNA was checked by the miRWalk database. Result The IFI۶ gene was found to have a significantly high expression level in breast cancer with an acceptable LogFC (۵.۳۷۳۸۳) and adj.P.Val (۱.۱۱E-۱۶). Founded SNP considered as a deleterious SNP obtained from the SIFT database named rs۹۲۴۹۵۹۱۷۹, and HOPE database analysis revealed a change in Glycine amino acid (G) to Aspartic acid (D) at position ۳۲. The mutant residue is bigger than the wild-type residue and, the wild-type is more hydrophobic than the mutant type. The mentioned gene IFI۶ is targeted by hsa-miR-۴۲۹۸ with an interaction energy of (-۳۴.۵), a seed region of (۱), and it affects the ۳’UTR region based on the miRWALK database. HELLPAR lncRNA confirmed by the lncRREASEARCH database discovered to be associated with the IFI۶ gene. Eventually the interaction between the mentioned lncRNA and its related miRNAs was studied using the lncbase v۳ site. Conclusion Microarray analysis of the IFI۶ gene demonstrated its significant overexpression in breast cancer and revealed that it can be suggested as a biomarker for therapeutic purposes. The associated interaction between miRNA and lncRNA can also provide a proper insight into the cancer progression.

کلیدواژه ها:

Breast ، cancer ، IFI۶ ، has-mir-۴۲۹۸