Investigating the effect of anti-Toxoplasma gondii antibodies on breast cancer

Toxoplasma gondii is an obligate intracellular parasite that exists mostly in tropical and subtropical regions. The final host of this parasite is the cat and its intermediate host is warm-blooded vertebrates. The anticancer effect of some Parasites indicates that it may be possible to control cancer due to the presence of common antigens between parasites and cancer cells. Method: In this case-control study, the study population includes a ۴T۱ cell line. ۴T۱ cancer cell line was purchased. On the other hand, serum samples from different referring patients in Clinical laboratories of Urmia city to investigate toxoplasmosis infection were collected All collected sera were tested using an ELISA kit To detect IgG antibodies against Toxoplasma, ۴T۱ cell line were cultured in RPMI-۱۶۴۰, then with ۱۰?tal bovine serum (FBS) with ۱% The antibiotic mixture containing ۱۰۰ international units per milliliter of penicillin is incubated in a humid environment. ۴T۱ cancer cells after harvesting from The culture medium were washed with trypsin and PBS. After counting the cells for each cell line, ۱۶ cells Tubes containing ۱۰۵ The cells were launched. The tubes were ۱ to ۸ As a case group, they were treated with positive toxoplasma serum. Tubes ۹ to ۱۶ were treated with Toxoplasma negative serum As a control group after ۱ hour of incubation, The tubes were washed ۳ times and then received secondary antibodies (Goat Anti Human IgG-FITC from Bio Legend Company)The conjugate with fluorescein isothiocyanate was added were analyzed with the surface of cells using flow cytometry method. Result: The reaction of Toxoplasma positive serum with the surface ۴T۱ breast cancer cells Reactivity between Toxoplasma positive serum And Toxoplasma serum was negative with the ۴T۱ cell surface It was investigated using flow cytometry method. The results showed that the cells reacted positively with Toxoplasma serum. It was stained with fluorescein isothiocyanate and transferred M۲ region in the flow cytometry graph while untreated cells remained in the M۲ region. As a result, Toxoplasma is positive The serum showed a specific reaction with ۴T۱ cells. Conclusion: Mouse breast cancer cells (۴T۱) while the reaction of these cells With negative toxoplasma, my serum was weak. These results show that the anti-toxoplasma antibody reacts with it. The reaction of anti-toxoplasma antibodies with The level of breast cancer cells may be the reason Common antigens between this parasite and cancer Cells in line with this hypothesis, in previous studies, The existence of common antigens between certain parasites and cancers have been shown.

کلیدواژه ها:

Toxoplasma gondii ، antibodies ، breast cancer