Esophageal cancer

Background Esophageal cancer is a type of cancer that occurs in the esophagus, the tube that carries food from the mouth to the stomach. It can be classified into two main types: squamous cell carcinoma and adenocarcinoma. Symptoms may include difficulty swallowing, weight loss, chest pain, and coughing. Treatment options typically include surgery, chemotherapy, radiation therapy, or a combination of these approaches. In this article with different analysis, the aim is to find biomarkers for this cancer. Methods Microarray analysis was performed on GSE۱۶۱۵۳۳ in the field of esophageal squamous cell carcinoma from the GEO database. MMP۱ gene was selected as a gene with significant increase in expression after checking with ENCORI and GEPIA۲ databases,. The signaling pathways in which the MMP۱ gene was active were checked by KEGG and Reactome databases. After analyzing the SNPs related to the said gene through the SIFT, miRNASNP and dbSNP databases, After analyzing SNPs related to the mentioned gene through SIFT, miRNASNP and dbSNP databases, A SNP in ۳ UTR named rs۹۰۹۷۹۹۵۳۷ was selected and a SNP in coding sequence were selected and their effects on the target gene were analyzed with the Hope database. The protein - protein interaction of the gene was analyzed by STRING database. The interaction between the desired gene and its related miRNAs was checked by miRWalk database. Conclusion Protein MMP۱ is involved in the degredation of extracellular matrix in disease processes, such as arthritis and metastasis. After microarray analysis on MMP۱ gene, the result of this study was that the said gene has a significant increase in expression on esophageal cancer and can be a biomarker in this cancer. The studies conducted on the protein-protein interaction of this gene showed that the target gene interacts with a significant number of other genes, and there is a possibility that these genes also affect the studied cancer. Results The deleterious SNP obtained from the SIFT database named rs۳۷۰۳۴۹۶۴۶ after checking by the Hope database showed that it had a change in the ۱۳۷th amino acid position and this change was from Alanine to Glycine The post-mutation state of this amino acid is smaller than the normal state, and the mutated state is also more hydrophobic than the normal state. The mutated is located in a domain that is important for the activity of the protein and in contact with another domain that is also important for the activity. The interaction between this domains could be disturbed by the mutation, which might affect the function of the protein. After analyzing the desired gene with its related miRNAs, hsa-miR-۳۷۰-۳p was selected as the miRNA affecting the ۳UTR region.

کلیدواژه ها:

esophageal squamous cell carcinoma ، Diagnostic markers ، Cancer genomics