Investigating the Regulatory Role of miRNA, SNP, and lncRNA on TP۵۳BP۲ Expression in MAP۳۰-Treated Bladder Cancer Cells Using Bioinformatics

Bladder cancer ranks among the top ten most common cancers worldwide , presenting a complex disease associated with elevated morbidity and mortality rates in the absence of optimal treatment. The importance of early diagnosis and personalized treatment plans cannot be overstated, as they are crucial for achieving favorable outcomes. MAP۳۰, also known as Momordica antiviral protein ۳۰ kDa, is a type I ribosome-inactivating protein (RIP) renowned for its antibacterial and antitumor properties . A biomarker is a measurable indicator of health or disease that predicts clinical outcomes and aids treatment, offering faster, cheaper, and more efficient screening, diagnosis, and personalized care. The primary objective of this study is to identify biomarkers for the early diagnosis of bladder cancer. MATERIALS AND METHODS: Bladder cancer (BC) cells were divided into two groups: the control group (untreated cancer cells) and the MAP۳۰-treated group. The expression of the TP۵۳BP۲ gene in both groups was measured using the Affymetrix Human Gene Expression Array. Data obtained from GSE۲۵۶۲۹۲ were analyzed using R programming, allowing for a comparative examination of gene expression between the two cell groups. In the miRNASNP database, we searched for microRNAs and SNPs associated with BC. The identified microRNA was subsequently examined in the miRWalk database, resulting in a list of genes influenced by this microRNA. Using R programming, common genes were identified between the set of genes affected by the microRNA and those expressed in BC. Furthermore, a list of long non-coding RNAs (lncRNAs) implicated in BC was obtained from the LncRNADisease v۲.۰ database. The interaction between these lncRNAs and the TP۵۳BP۲ gene was then investigated using the LncRRIsearch database. RESULTS: Based on data obtained from miRNASNP, both rs۱۱۵۷۶۹۱۶۹ as a single nucleotide polymorphism (SNP) and hsa-miR-۴۴۵۷ are implicated in bladder cancer. The TP۵۳BP۲ gene was identified as a common gene between the set of genes affected by hsa-miR-۴۴۵۷ and those expressed in bladder cancer. Additionally, data from the miRWalk database indicate that the microRNA hsa-miR-۴۴۵۷ interacts with the TP۵۳BP۲ gene. Finally, according to data from LncRNADisease v۲.۰ and LncRRIsearch, H۱۹ has been identified as a long non-coding RNA (lncRNA) that interacts with the TP۵۳BP۲ gene, which plays a significant role in bladder cancer. CONCLUSIONS: The results indicate a significant increase in TP۵۳BP۲ gene expression in bladder cancer cells treated with MAP۳۰, highlighting the anti-cancer activity of this compound. Furthermore, based on the findings of the current study, it can be concluded that SNP rs۱۱۵۷۶۹۱۶۹, hsa-miR-۴۴۵۷, and lncRNA H۱۹ play critical roles in regulating gene expression in bladder cancer and may serve as valuable biomarkers for the early diagnosis of this disease.

کلیدواژه ها:

TP۵۳BP۲ ، rs۱۱۵۷۶۹۱۶۹ ، hsa-miR-۴۴۵۷ ، HRG-AS۱ ، MAP۳۰