The impact of copper nanoparticle on breast cancer: An in vitro model

: Breast cancer is the most common malignancy among women, affecting about ۱.۳ million women annually. Despite advances in cancer treatment, it remains a leading cause of cancer-related death among women. Current treatments have limitations, and there is a need for effective, biocompatible, and cost-effective therapeutic agents. Nanomaterials, particularly nanoparticles, show promise in enhancing cancer therapies due to their unique properties. Copper nanoparticles (CuNPs) have demonstrated high toxicity against tumor cells, including those associated with breast cancer. They have the potential to induce programmed cell death in breast cancer cells. Further research is needed to assess the impact of CuNPs on breast cancer cells and elucidate the mechanisms underlying cell death. methods: MCF-۷ and MCF-۱۰ cells were cultured and treated with copper nanoparticles (CuNPs) in specified doses ranging from ۵ to ۲۰۰ μg/ml. The effects on cell viability, apoptosis, cell cycle, and gene expression were evaluated using MTT assay, flow cytometry, and real-time PCR. Statistical analysis was performed using GraphPad Prism ۱۰ software by performing T-test and one-way ANOVA. results: MCF-۷ and MCF-۱۰ cell viability was reduced in a dose-dependent manner after treatment with CuNPs for ۲۴ hours. Exposure to ۳۵.۴ µg/ml and ۸۵ µg/ml CuNPs resulted in ۵۰% ± ۱.۲ reduction in cell viability for both cell lines. Moreover, flowcytometry results demonstrated that the treatment with CuNPs led to ۱۹.۹?rly apoptosis and ۳۴.۶% late apoptosis for MCF-۷ cells, and ۲۶.۳?rly apoptosis and ۲۸.۴% late apoptosis for MCF-۱۰ cells. On the other hand, at the IC۵۰ concentration of CuNPs, the peak value of SubG۱ confirmed programmed cell death but there were no significant differences in the S and G۲ phases compared to the control groups. At the genes level, BCL-۲ gene expression significantly decreased in MCF۱۰ and MCF۷ cells, while BAX gene expression increased with higher CuNPs concentrations. Therefore by considering all the results, CuNPs can promote apoptosis in breast cancer cell lines in invitro model. Conclusion: The findings of this study suggest that copper nanoparticles (CuNPs) may exhibit anti-tumor properties in MCF-۷ and MCF-۱۰ cells by potentially modulating the BAX and BCL-۲ genes and induce programmed cell death. as a result, Perhaps by the advances in cancer Nano therapy in future copper Nano particles can be an eligible candidate for breast cancer treatment. However, further research to validate and elucidate the precise underlying mechanism responsible for the anti-tumor effects of CuNPs in an In vivo and clinical models is needed.

کلیدواژه ها:

breastcancer ، MCF-۷ ، MCF-۱۰ ، copper ، nano-particle