Evaluation of the effect of alginate-basedscaffolds containing graphene oxide nanoparticles on glialcell lines

Background: Various research is being done in the field ofdeveloping different methods of nerve cell repair all over theworld. Therefore, neural tissue engineering researchers inventedscaffolds that can help repair neural tissue according to itsstructure and components. Scaffolds should have features suchas biocompatibility, increased cell viability, differentiation, productionof extracellular matrix, etc., with natural bone tissue,three-dimensional structure with internal pores related to andthe chemical surface of one of the therapeutic approaches. alginatecan fill the cavities of the damaged brain and spinal cordand provide a framework for the regrowth and connection ofaxons.Materials and Methods: Biodegradable scaffolds based onalginate-containing nanoparticles were synthesized in concentrationsof graphene oxide nanoparticles (۰.۵, ۱, and ۱.۵). Andthe destruction test was done to characterize them to check thequality of the synthesized scaffolds, then the glial cells werecultured on them, and the SEM test (to check the morphology ofthe cells on the scaffold) and the MTT test (to check the toxicityeffect of the scaffolds) containing nanoparticles on the cells ondays ۱, ۳ and ۷).Results: The results of the MTT test showed that the biodegradablescaffolds based on alginate nanoparticles in differentconcentrations of graphene oxide (۰.۵, ۱, and ۱.۵) on days ۱,۳, and ۷ show that the scaffold based on alginate-containing nanoparticles synthesized with a new formulation on the cellline It has a neurological effect and increases cell growth andproliferation compared to the control sample.Conclusion: Biodegradable scaffolds based on alginate containinggraphene oxide nanoparticles increase the viability ofcells and can become a suitable substrate for the growth andproliferation of glial cells.

کلیدواژه ها:

alginate ، graphene oxide ، nanoparticles ، glialcellline