Molecular signature analysis of highthroughput data reveals fusobacterium nucleatum's involvementin the development of colorectal cancer

Background: Fusobacterium nucleatum is an anaerobic gramnegativebacteria found in the mouth, gut, and female vaginalcanal. F. nucleatum has been linked to several illnesses, likecolorectal cancer. Colorectal cancer (CRC) is a major globalhealth concern since the colon is an important digestive organ.F. nucleatum has been linked to both the onset and progressionof colorectal cancer. Evidence suggests that this bacterium adheresto and invades colon cancer cells, fostering the developmentof biofilms that shield malignant cells from the immunesystem and treatment and promoting cancer cell proliferationand metastasis. Interacting with immune cells, causing inflammation,and changing the gut microbiome are all ways that F.nucleatum has been demonstrated to promote tumor developmentand invasion. Even if the precise methods by which thisbacterium promotes cancer formation and progression remainunknown, this study's results indicate the molecular signatureto know much about the cancer growth process of F. nucleatum.Materials and Methods: Bioinformatics tools investigatedthe NCBI SRA repository's SRP۳۱۳۷۸۰ dataset. Differentiallyexpressed genes (DEGs) in untreated and F. nucleatum-treatedcell lines were identified using the DEseq۲ package in R. Theonline Enrichr tool was used to query the KEGG (Kyoto Encyclopediaof Genes and Genomes) database for gene-relatedpathways. Cytoscape displayed the pathway information.Results: Compared to controls, treatment groups significantlyelevated ۶۳ genes. According to functional enrichment analysis,these genes are involved in multiple pathways and contribute tocancer development. Engaging with immune cells, the genes(CXCL۳, CXCL۲, CXCL۱, CXCL۸, JUN, NFKBIA, BIRC۳,ICAM۱, TNFAIP۳, and CCL۲۰) are considerably elevated inthe progression of cancer.Conclusion: Our research identifies possible gene-related pathwaysthat need further exploration and sheds information on themolecular impacts. F. nucleatum on colorectal cancer cell lines.

کلیدواژه ها:

Fusobacterium nucleatum ، Colorectal cancer ، Bioinformatics