MicroRNA regulatory network in FOXR۲gene

Introduction: FOXR۲ identified as a potent and ubiquitousoncogene that is epigenetically activated across the majority ofhuman cancers.FOXR۲ is a transcription factor located on theX chromosome and normally restricted to the testis. MicroRNAsare a family of small non-coding RNAs that play regulatoryroles by targeting mRNAs also regulate a wide array ofbiological processes including carcinogenesis. Compelling evidenceshave demonstrated that miRNA expression is dysregulatedin human cancer through various mechanisms, includingamplification or deletion of miRNA genes, abnormal transcriptionalcontrol of miRNAs, dysregulated epigenetic changes anddefects in the miRNA biogenesis machinery.MicroRNAs mayfunction as either oncogenes or tumor suppressors under certainconditions. An increasing number of studies have identifiedmiRNAs as potential biomarkers for human cancer diagnosis,prognosis and therapeutic targets.Methods: In this research, new microRNAs in FOXR۲ genewere predicted by bioinformatics methods. SSC profiler programwas utilized to predict the secondary structures.UCSCgenome browser database was used to analyze the conservationstatus. Furthermore, theFOXR۲-miRNAs prediction wasalso performed by using MatureBayes online tool. In addition,RNAFOLD online software was used for approximate predictionof the secondary structure.Results: The results showed that there is a miRNA stem-loopsequence in the FOXR۲ gene that has not been reported so far.To prove the realness of these structures predicted by bioinformaticsmethods, there is a need to perform experimental laboratoryevaluations that yield results from NGS reads that do notexpress the predicted miRNA.Conclusion: The method used in this study has previously beenused for the bioinformatics identification of miRNAs, whichwere confirmed to exist after prediction using experimentalmethods. Therefore, it seems that there are micro-RNAs in thegene structure that can explain part of the complexities of genefunction.

کلیدواژه ها:

Cancer ، miRNA ، FOXR۲ ، bioinformatics