Integrated Bioinformatics Analysis RevealsKey Candidate Genes and pathways and MicroRNAsin Non-small cell lung cancer

Background: Non-small cell lung cancer (NSCLC) is the secondmost common cancer worldwide. Therefore, according to the importanceof this disease, finding the genes and cellular pathwaysinvolved in this disease and finding treatment opportunities haveled us to see these effective genes using bioinformatics.Materials and Methods: The bioinformatics analysis mostlyinvolved the Gene Expression Omnibus (GEO). First, wescreened DEGs based on the Transcriptome Analysis Console(TAC). Next, the protein-protein interaction (PPI) network ofthe DEGs was built in the STRING database. Then, hub geneswere screened through the Cytoscape. Additionally, by usingEnrichr, we found the cellular pathways that our hub geneswere involved in, and by using miRDB, we found the appropriatemiRNAs for them.Results: We ended up with ۴۳۸۰ DEGs, including ۱۶۳۷ upregulatedand ۲۷۴۳ downregulated DEGs. From the PPI, we filteredout ten hub genes, and these genes were significantly upregulatedin NSCLC samples, findings consistent with the expressionvalidation Results: Also, survival analysis showed high-levelgene expression levels of NEIL۳, GMPS, RFC۴, CDK۱, EXO۱,BRCA۱, PSMD۱۴, MAD۲L۱, CDC۶, and CCNB۱ were connectedwith the poor overall survival of NSCLC patients. Themost important cellular pathways in which these genes wereinvolved were cell cycle checkpoints, cell cycle, and G۲/Mcheckpoints. We found miRNAs for our own hub genes withthe highest score, including hsa-miR-۶۵۶-۳p for NEIL۳, hsamiR-۵۰۱۱-۵p for CDK۱, hsa-miR-۳۴۰-۵p for EXO۱, hsa-miR-۱۲۵a-۳p for BRCA۱, hsa-miR-۶۰۷ for PSMD۱۴, hsa-miR-۴۹۳-۳p for MAD۲L۱, hsa-miR-۳۱۱۵ for CDC۶, and hsa-miR-۵۴۸nfor CCNB۱.Conclusion: The present study screened out the essential genesand pathways related to NSCLC pathogenesis, which couldprovide new insights for the future molecular targeted therapyand prognosis evaluation of NSCLC. The influential genes wereidentified based on betweenness and ۱۰ genes were identifiedusing Enrichr, the cellular pathways in which these genes hadthe most significant impact. MiRNAs were predicted for them,but for GMPS and RFC۴ no Human miRNA is predicted to target.We can conduct various research to find miRNA for twogenes, and by using additional studies and in vivo and in vitrostudies, we can find new treatment methods for this cancer

کلیدواژه ها:

NSCLC ، GEO ، miRNA ، TAC