Nanoparticles of Silymarin-Tin (IV) mediatedanti-colorectal cancer activity via enhancing the hydrophobicnatural compound delivery

Background: Silymarin (SM) exhibits potential therapeutic effectsdue to having antioxidant activity. However, the low solubilityand bioavailability of SM restrict its biological performance.This study aimed to develop a novel nanoformulationcomposed of SM and dimethyltindichloride and investigate theeffect of SM Silymarin-loaded Sn nanoparticles on cancer cellgrowth and survival to overcome this limitation.aterials and Methods: SM-Sn complex was synthesized andthen characterized using X-Ray Diffraction (XRD), TransmissionElectron Microscopy (TEM), Fourier Transform Infrared(FTIR) and EDS-MAP analysis. Afterward, the SW۴۸۰ colorectalcancer cell line was treated with different SM and SMSncomplex concentrations. We examined cell viability usingMTT assays, apoptosis analysis. In flow cytometry, ROS levelsand cell cycle were measured intracellularly. A colony formationassay was performed to evaluate the colonization abilityof SW۴۸۰ cells. Real-time PCR was also used to analyze geneexpression.Results: The results of this study revealed the effectivenessof the SM-Sn complex in decreasing SW۴۸۰ cell viability byinducing cell death-associated mechanisms. We found that theSM-Sn complex increases intracellular ROS level. It was alsorevealed that the SM-Sn complex induces cell cycle arrest andthe expression of apoptotic genes. In addition, the SM-Sn complexcould effectively hinder SW۴۸۰ cells from constitutingcolonies.Conclusion: We conclude that the use of silymarin-tin (IV)nano-complex enhanced delivery of SM could be considered anefficient option for inhibiting colorectal cancer cell proliferationand survival.

کلیدواژه ها:

Colorectal cancer ، Silymarin ، Tin (IV) ، Complex ، Cell survival