Predicting the regulatory role of hsa-miR-۳۳۸-۳p and its interaction by bioinformatics

Background: Prostate cancer (PCa) is a common malignancyin the urinary system and is a leading cause of cancer death inmales.۱ Most of the early-stage PCa are hormone dependent,and only a few belong to hormone-refractory prostate cancer(HRPC). MicroRNAs (miRNAs) deregulation is common inhuman cancers, and understanding how it impacts in PCa is ofmajor importance. MiRNAs are mostly downregulated in cancer,although some are overexpressed, playing a critical role intumor initiation and progression. We aimed to identify miRNAsoverexpressed in PCa and subsequently determine its impact intumorigenesis.Material and Materials and Methods: In this project, aftermiRNA target gene prediction by mirwalk database, we analyzedTCGA RNA-seq data and predicted gene expression patterns(۳, ۴).Results: The results of mirwalk analysis show the strong interactionof hsa-miR-۳۳۸-۳p with PACRGL, and the results ofprostate cancer genomics data analysis show a significant decreasein the expression of PACRGL in the tumor sample comparedto the normal sample. The decrease in hsa-miR-۳۳۸-۳pexpression in stage III samples is also higher than in primarytumor samples.Conclusion: According to the location of hsa-miR-۳۳۸-۳pbinding to PACRGL and this negative relationship betweenincreased hsa-miR-۳۳۸-۳p expression in tumor samples anddecreased PACRGL expression in tumor samples, as well asdecreased PACRGL expression in nodal metastasis samples andincreased hsa-miR-۳۳۸-۳p expression in nodal metastasis samples,regulation of PACRGL expression by hsa-miR-۳۳۸-۳p canbe predicted. In addition, according to the results of the TCGAwebsite, it is predicted that the reduction of gene expressionplays a role in the progression of cancer to nodal metastasis and in the development of primary tumors

کلیدواژه ها:

PACRGL ، hsa-miR-۳۳۸-۳p ، prostate ، Bioinformatics