Effect of Ivermectin on induction of apoptosisin human keratinocyte cell line (HaCaT)

Objectives: Cancer is one of the most common complicationsin the world and cutaneous squamous cell carcinoma (cSCC) isamong the most prevalent cancers. Most cSCCs can be treatedby surgery, but we need other treatments for few resistant cases.HaCaT is a popular cell line for skin cancer studies. The aim ofthe present study was to investigate the effect of different dosesof ivermectin on the cell viability of HaCaT cancer cells.Materials and Methods: HaCaT cells were cultured in DMEMmedium containing ۱۰% FBS and treated with ۰.۲, ۰.۴, ۰.۸, ۱.۵,and ۸ μM concentration of ivermectin and then ۷۲ hours laterwere evaluated by MTT assay for their viability. The morphologyof the cells was also examined by Giemsa staining usinginvert microscopy. Also, the expression of Bax, Bad, BCL-۲genes was evaluated via Real-Time PCR. Data were analyzedusing SPSS version ۲۵.Results: Using MTT assay, IC۵۰ of ivermectin on HaCaT cellswere determined to be ۱.۵ μM. ۰.۲ and ۸ μM had the lowest andhighest apoptotic effect on HaCaT cells. PCR results showedelevated Bax and Bad and reduced BCL-۲ gene expression.Conclusion: Ivermectin was able to exert a good killing and apoptoticeffects on cancer cells. Therefore, ivermectin can probablybe used as an anticancer agent in modern cSCC therapies.

کلیدواژه ها:

HaCaT cell line ، Ivermectin ، Apoptosis ، MTT