Targeting genes for miR-۱۴۲-۵p in pancreaticcancer cells

Introduction: Pancreatic cancer is a disease which has beenstill remained as one of the most lethal cancers without anyspecific asymptomatic until high stages and thus without anyspecial treatment. Risk factors for this disease are smoking,obesity, type ۲ diabetes and family history. PDAC (pancreaticductal adenocarcinoma) is stated as the most common and aggressivesubtype of pancreatic cancer with the highest mortalitybecause of poor response to several treatments. Nowadays genetherapy is under consideration to look for a treatment for pancreatic cancer a case in point is tumor suppressor MicroRNAs.MicroRNAs (miRNAs/miRs) are a group of small, non-coding,regulatory RNAs which are ~۲۲ nucleotides in length. Recentlythey have become more popular because of their effect on targetgenes in cancer cells. Tumor suppressor miRs can regulate theproliferation and growth of cancer cells through down regulatingoncogenes which leads to inhibiting migration and proliferationof cancer cells. Researches show that miR-۱۴۲ -۵p hasbecome low in pancreatic cancer cells. In our study we willdefine two target genes for mir-۱۴۲-۵p.Method: miR-۱۴۲-۵p was selected based on expression data inpancreatic cancer. Target genes were chosen through bioinformaticstools Target Scan, miRDB and miRWalk based on Sitesmatching in the miRNA seed region and cumulative weightedcontext++ score.Result: Zfpm۲ and Rap۱a were chosen based on their scores. Intarget scan ZFPM۲ has ۳, ۸mer conserved sites and -۰.۸۴ cumulativecontext score and RAP۱a has ۱, ۸mer conserved site and-۰.۳۸ cumulative context score which are acceptable.Conclusion: In Conclusion: , Based on bioinformatics analysismiR۱۴۲-۵p can be considered as a potential tumor suppressorby targeting oncogenes in PDAC. As predicted by computationalinvestigation, two transcripts Zfpm۲ and RAP۱a are twocritical targets in cell-cell junction formation and cell polarity.With considering their score by matching ۳ UTR of gene withmiR seed region, studying on these genes and miR-۱۴۲ can leadto find a promising way for treating PDAC.

کلیدواژه ها:

Gene therapy ، miRs ، pancreatic cancer ، PDAC