Examining the association between theHox transcript antisense intergenic RNA variant and therisk of colorectal cancer

Background: Multiple studies have worked on the genetic variations,especially single nucleotide polymorphisms (SNPs) andgastrointestinal disorders. Among numerous gastrointestinalcarcinomas, colorectal cancer (CRC) and related genetic variationshave been concentrated. Even though several researchprojects have looked into the association between the susceptibilityof CRC and SNPs, the molecular causes of CRC arenot fully understood. In the current investigation, we looked atthe association between the Iranian population’s LncRNA Hoxtranscript antisense intergenic RNA (HOTAIR) polymorphism,rs۱۸۹۹۶۶۳, and the risk of CRC.Materials and Methods: In this research, genotyping ofrs۱۸۹۹۶۶۳ polymorphism, has been done for both the case andcontrol groups by using the Tetra-primer Amplification RefractoryMutation System- Polymerase Chain Reaction (TPARMS-PCR) method. For confirmation of our results, we havedone sequencing on some samples.Results: In the present study, our investigations about thers۱۸۹۹۶۶۳ polymorphism and susceptibility of CRC in theIranian population demonstrated an association between thers۱۸۹۹۶۶۳ SNP and CRC risk in the dominant and over-dominantmodels of inheritance. The sequencing results confirmedour investigations in current research.Conclusion: According to our study about the association betweenSNPs and CRC risk, we verified that rs۱۸۹۹۶۶۳ polymorphism inthe LncRNA HOTAIR gene is associated with susceptibility toCRC. Numerous studies are needed to confirm our results.

کلیدواژه ها:

Single nucleotide polymorphism ، lncRNA ، colorectalcancer ، HOTAIR