Investigating the growth and behavior of glialcells on alginate scaffolds containing graphene quantumdot nanoparticles

Background: Tissue engineering is the science of tissue designfor healing and repairing tissue damage. Tissue engineering ofthe nervous system using neural scaffolds is one of the therapeuticapproaches to replace damaged nervous tissue. For thispurpose, the alginate scaffold can fill the holes in the injuredbrain and spinal cord and provide a framework for regrowthand connection.Materials and Methods: After synthesis, alginate-based biodegradablescaffolds containing nanoparticles in concentrationsof graphene quantum dots (۰.۵, ۱, and ۱.۵) and determining itsproperties by degradation test to check the quality of synthesizedscaffolds, nerve cells were cultured on them. And to investigatethe morphology of the cells on the scaffold, an SEMtest, and an MTT test were performed to investigate the effectof the toxicity of scaffolds containing nanoparticles on the cellson days ۱, ۳, and ۷.Results: The results of the MTT test showed that the biodegradablescaffolds based on alginate nanoparticles in differentconcentrations of Graphene quantum dots (۰.۵, ۱, and ۱.۵) ondays۱, ۳ and ۷ show that the scaffold based on alginate containsnanoparticles synthesized with a new formulation on the categoryIt has an effect on nerve cells and increases the growthand proliferation of cells compared to the control sample. Synthesizedalginate scaffolds for safe and successful use in clinicalenvironments, biomaterials for neural tissue engineering withproperties such as biocompatibility, biodegradability, new cellgeneration, and nerve conduction, scaffold pore structure, andshowed themselves.Conclusion: By examining the results of the MTT test, it canbe concluded that the scaffolds containing the synthesized graphenequantum dots nanoparticles can optimize the substratefor the growth of glial cells and will increase the rate of cellgrowth and proliferation

کلیدواژه ها:

Alginate ، Graphene Quantum Dot ، Nanoparticles ، Scaffold