Evaluation of Anti-leishmanial Effect of Selenium Nanoparticles on Leishmania major Promastigotes In Vitro
محل انتشار: میکروبیولوژی بالینی و عفونت، دوره: 9، شماره: 3
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 163
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استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_JCMI-9-3_004
تاریخ نمایه سازی: 28 تیر 1402
چکیده مقاله:
Background: Cutaneous leishmaniasis (CL) remains a major health threatening disease in Iran and
many countries around the world. Antimony compounds are currently used to treat CL. Due to the side
effects and high resistance, the use of alternative therapies, especially the use of nanoparticles, has been
considered by researchers. The aim of this study was to investigate the anti-leishmanial activity of selenium
nanoparticles (SeNPs) on Leishmania major in vitro.
Methods: In this experimental study, the anti-leishmanial activity of the SeNPs was evaluated at
concentrations of ۱.۲۵, ۲.۵, ۵, ۱۰, ۲۵, ۵۰, and ۱۰۰ μg/mL at exposure times of ۲۴, ۴۸, and ۷۲ hours on
۱۰۶ live parasites. Then, the number of live parasites was counted by trypan blue using a neobar slide
and light microscope (Hemocytometer method). Glucantime and distilled water were considered positive
and negative controls, respectively. Then, ۵۰% inhibitory concentration (IC۵۰) values were calculated by
SigmaPlot™ ۱۳ software. All reactions were performed in triplicate, and the results were considered as
average.
Results: The results of this study revealed that all concentrations of SeNPs have anti-leishmanial activity.
The concentration of ۱۰۰ μg/mL of SeNPs had the highest anti-leishmanial effect (۱۰۰%) after ۷۲ hours of
exposure. Further, the IC۵۰ content of SeNPs on L. major after ۲۴, ۴۸, and ۷۲ hours was calculated to be
۴۲.۷۶, ۳۴.۵۳, and ۲۲.۶۹ μg/mL, respectively.
Conclusions: The results indicated that SeNPs in different concentrations has an inhibitory effect on the
growth of L. major. However, further investigations are required to determine the efficacy of SeNPs in vivo.
کلیدواژه ها:
نویسندگان
Roghayeh Norouzi
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Farzaneh Mirzaei
Department of Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Abolghasem Siadatpanah
Ferdows School of Paramedical and Health, Birjand University of Medical Sciences, Birjand, Iran
Seyed Jafar Adnani Sadati
Department of Microbiology Immunology and Parasitology, School of Medicine, Qom University of Medical Sciences, Qom, Iran- Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran