Evaluation of ZmNAC۱۰ gene sequence and protein structure of this gene to evaluate salinity stress resistance in plants

Corn is one of the most important cereals of American origin after wheat and has the largest agriculturallands in the world.This plant contains nutrients and can be a good source of fiber. Due to the value of thisplant, recognizing the genes involved in the path of resistance to stress is one of the most important issues ofthis plant. Irrigation method, environmental factors and medical stresses on it. To better understand andunderstand the molecular mechanism for resistance to stress, the gene and expression of ZmNAC۱۰ werespecifically studied. NACs are one of the largest family of plant-specific transcription factors (TFs) and playa key role in growth and stress responses. The sequence of this gene is highly similar to the sequence ofreceptor kinases (high-affinity cell surface receptors). In this study, ZmNAC۱۰ gene was studied usingbioinformatics sources and primers were designed to amplify this gene. Then, its cDNA was made by reversetranscriptase enzyme and used as a template for ZmNAC۱۰ gene amplification in PCR reaction. ZmNAC۱۰gene expression was lowest in stems and roots and highest in leaves. During the PCR reaction, a ۵۶۱ bpfragment of cDNA and a ۱۴۴۰ bp fragment of genomic DNA were amplified. This gene encodes a protein of۱۸۷ amino acids that has a region with a kinase sequence. By analyzing the molecular structure of thetranscribed protein, the isoelectric point and the molecular weight were obtained, and also based on thehomologous modeling, its spatial shape showed that this protein has a regular structure.

کلیدواژه ها:

Molecular mechanism ، Resistance to stresses ، Kinases Receptor ، Homologous modeling