Chronic kidney disease affects more than ۱۰% of the world population. ۹۰% of all Cases are attributed toglomerular disease. The most sever forms of kidney diseases are often associated with irreversible damageto glomerular podocytes -highly specialized epithelial cells that encase glomerular capillaries and regulateremoving toxin and wasted components from the blood. As a consequence, finding signaling pathwaysrelated to the podocyte damage by bioinformatics approaches is essential. In this study we focused on geneexpression profiling in damaged podocyte cells and investigated signaling pathways associated with thismatter. At first, we selected suitable studies from GEO database (GSE۱۵۱۶۹۰). This dataset consists of threegroups of glomerulonephritis with three difference times (W۰, W۱, W۵) and analyzed with GEO۲R tool.Then uploaded the up and down regulated genes to VENNY version ۲.۱.۰ tool and selected processed incommon genes between up/down regulated groups to examine the signaling pathways in the DAVID databaseand the KEGG library then loaded the genes involved in biological process, cell component and molecularfunction. Finally, we used STRING database to select protein interaction.Results showed that ۱۶۷ genes up regulated and ۱۹۰ genes were down regulated. Among those up regulatedgenes most of them were expressed in focal adhesion, PI۳K-Akt signaling pathway, cell adhesion molecules(CAMs), cytokine-cytokine receptor interaction, in other hand metabolic and TGF-β signaling pathway wereobserved in down regulated genes. JacK۳, Col۱a۱, Fn۱, Ptprc, Pdgfb, Pdgfbr genes were up regulated andthe results also obtained that Acadsb, Acmsd, Acsm۳, Bmp۴, Dmgdh were down regulated. By this analysisand results we observed the genes that identified in podocyte cells damages that highly related to cytoskeletonstructure denature, metabolic pathways.