Study of the anti-cancer effect of oxypeucedanin methanolate purified from Ferulago trifida Boiss plant on A۵۴۹ lung cancer cell line

Introduction: Lung adenocarcinoma is the most primary histologic subtype of non-small cell lung cancer (NSCLC). oxypeucedanin methanolate, a member of furanocoumarin, is a naturally occurring compound, which is isolated from Ferulago trifida Boiss, an endemic species in north- west of Iran. We attempt to uncover the capacities of oxypeucedanin methanolate to induce apoptosis and autophagy in NSCLC cells, as well as the underlying mechanism involved in this process. Methods: The effect of oxypeucedanin methanolate on cell viability was evaluated on A۵۴۹ cells by MTT assay. Flow cytometry assay was used to detect cellular apoptosis. Expression levels of BAX, caspase ۳, BCL۲ and LC۳ in A۵۴۹ cells were measured by real-time quantitative reverse transcription-polymerase chain reaction (Real time qRT-PCR). Results: Oxypeucedanin methanolate inhibited A۵۴۹ cell proliferation in dose- and time- dependent manner, as evaluated by MTT assay. The total apoptosis rate was (۵.۴۶%) for A۵۴۹ cells not treated with oxypeucedanin methanolate. In contrast, the apoptosis rate was (۲۹.۶%) for A۵۴۹ cells treated with oxypeucedanin methanolate at the concentration of ۰.۴ mM. Real-time quantitative RT-PCR revealed that the mRNA expression of BAX, caspase ۳ and LC۳ were upregulated, while mRNA expression of BCL۲ was downregulated. Conclusion: Oxypeucedanin methanolate inhibits proliferation and it could induce apoptosis and autophagy of human non-small cell lung cancer cell line A۵۴۹.

کلیدواژه ها:

Non-Small Cell Lung Cancer (NSCLC) ، Lung adenocarcinoma ، Furanocoumarin ، Oxypeucedanin methanolate ، Apoptosis ، Autophagy