Principal component analysis of essential oil compounds of Rydingia michauxii at the dormant, vegetative and flowering stages

Rydingia michauxii belonging to the Lamiaceae family, is an herb used in Iranian traditional medicine. Rydingia is traditionally used for treating a number of diseases including diabetes, arthritis, gastric discomfort, headache and rheumatism. Various parts of R. michauxii (roots, leaves, stems and flowers) were collected at the three developmental stages (dormant, vegetative and flowering) in Kazeroun, Iran. The essential oil was isolated by a Clevenger–type hydro-distillation apparatus and chemical composition of essential oil was determined by gas chromatography/mass spectrometry (GC/MS). Principal component analysis (PCA) was conducted to evaluate the degree of phytochemical variations according to a correlation matrix of R. michauxii compounds at the three developmental stages. PCA indicated two components with ۱۰۰% of the total variance. The first and second components (PC۱ and PC۲) revealed ۵۶.۷% and ۴۳.۳% of the total variation, respectively. The PC۱ consisted of compounds including eugenol, eugenol acetate, carvone, geraniol, ۱,۸-cineole, myrcene, myrtenol, -cadinene and bornyl acetate. Moreover, the PC۲ included compounds such as trans- -bergamotene, -guaiene, methyl eugenol and linalool. Biplot analysis of the chemical compounds of PC۱ and PC۲ showed that dormant, vegetative and flowering stages are placed into three different groups representing the diversity in essential oil compounds during the growth stages.

کلیدواژه ها:

Correlation matrix of compounds ، Developmental stages ، Rydingia michauxii