Evaluation of changes by chromium pollutant in the red blood cells epaulet grouper (Epinephelus stoliczkae) in vitro

Chemicals and pollutants affect the properties of blood cells and provide useful information about the environment of organisms. The aim of the present study was to investigate the changes in the red blood cells (cellular and nuclear major axis, cellular and nuclear minor axis, cell and nuclear volume, cytoplasmic volume) of Oman Sea epaulet grouper (Epinephelus stoliczkae) under laboratory conditions exposion of chromium contaminant for ۲۱ days. For this purpose, three sub-lethal concentrations of chromium metal ۳.۶, ۷.۳۱ and ۱۴.۶ mg / l were selected for the treatment of epaulet grouper and blood samples were taken from the fish at the end of ۲۱ days. The major and minor axis of blood cells and nucleus were measured with micrometer of light microscope. Erythrocyte, nuclear and cytoplasm volume were calculated using by existing equations. The results of this study showed that there was a statistically significant difference between the parameters measured in the red blood cells of the control group and the groups treated with chromium pollutants (P <۰.۵). Factors investigated of red blood cell in epaulet grouper can be used to monitoring of chromium contamination.

کلیدواژه ها:

Red blood cell ، Fish ، Pollution