Bioinformatics study of hsa-miR-99a and LNCRNA ANRIL targeting pathways in acute lymphoblastic leukemia

Introduction & Objectives: Acute lymphoblastic leukemia is the most common cancer in children. Since the etiology of hematologic diseases is unknown, it has been attempted to investigate regulatory and functional genes such as LNCRNAs. ANRIL is one of the LNCRNAs that its expression, regulates its important adjacent genes, CDKN2A and CDKN2B (coding tumor suppressor proteins). The aim of this study was to analyze the bioinformatics pathway of LNCRNA ANRIL (CDKN2B-AS1) by targeting its specific microRNA in leukemia cancer.Materials & Methods: At first, studies of different articles revealed that ANRIL LNCRNA causes cancer by targeting different microRNAs in different cancers. hsa-miR-99a is one of the microRNAs that causes tumor growth through suppression by ANRIL. By confirming the role of hsa-miR-99a in leukemia cancer through the phenomiR database, this microRNA was analyzed by the miRbase database. Then, a variety of hsa-miR-99a target genes were searched in the MiRWalk database and the most relevant signal pathways from the KEGG database to the target genes and hsa-miR-99a were analyzed by using the DAVID bioinformatics database.Results: Results from the KEGG and phenomiR database indicatied that hsa-miR-99a regulates tumor suppressor genes by targeting CDKN2A and CDKN2B genes. On the other hand, hsa-miR-99a has been shown to be downregulated in leukemia, and by less targeting these two genes, it can cause cancer. As a result, ANRIL indirectly causes cancer by suppressing aging by the action of CDKN2A and CDKN2B on metastatic development.Conclusion: Bioinformatics of cancer pathways indicatied that ANRIL LNCRNA inhibited tumor suppressor genes by suppressing hsa-miR-99a. As a result, ANRIL LNCRNA is an oncogene gene and hsa-miR-99a is a tumor suppressor mir in the leukemia cancer pathway.

کلیدواژه ها:

leukemia ، LNCRNA ANRIL ، hsa-miR-99a ، CDKN2A ، CDKN2B