Design and construction of a new operator: A new attempt to improve protein expression under industrial condition

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 545

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شناسه ملی سند علمی:

CIGS15_229

تاریخ نمایه سازی: 13 بهمن 1398

چکیده مقاله:

SOS response system is one of the most studied cellular systems in many bacterial species. Almost more than forty-independent SOS genes are engaged in function of SOS system. Encoded proteins participate in some process like replication, mutagenesis, repair, and subsequently, response to any intra/extra-cellular stress that can harm to DNA structure or DNA replication. When there is no any inductive stress (normal growth conditions), expression of SOS genes is at a basic level, after induction, the level of expression exponentially rises. Induction of SOS response system contains more than 40-independent SOS genes that encode proteins engaged in the protection, repair, replication, mutagenesis and DNA metabolism. Under normal growth conditions, SOS genes are expressed at a basic level, as a result of induction, the expression significantly increased. In this study, we designed and analyzed a derivative operator compatible for a broad range of temperatures. In silico analysis of this study showed a very interactive construct that can attract effectively the lexA repressor. This study is a primary modelling study for creation of a new generation of vectors applicable in the industrial biotechnology.

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نویسندگان

Mohammad Saba Yousef Mardoukhi

Department of Genetics, Faculty of Science, University of Shahrekord, Shahrekord, Iran.

Ali Mohammad Ahadi

Department of Genetics, Faculty of Science, University of Shahrekord, Shahrekord, Iran.

Hoda AYAT

Department of Genetics, Faculty of Science, University of Shahrekord, Shahrekord, Iran.

Seyed Shahriar Arab

Department of Biophysics, Faculty of Biological Science, Tarbiat Modarres University, Tehran, Iran.