Design and construction of a new operator: A new attempt to improve protein expression under industrial condition

SOS response system is one of the most studied cellular systems in many bacterial species. Almost more than forty-independent SOS genes are engaged in function of SOS system. Encoded proteins participate in some process like replication, mutagenesis, repair, and subsequently, response to any intra/extra-cellular stress that can harm to DNA structure or DNA replication. When there is no any inductive stress (normal growth conditions), expression of SOS genes is at a basic level, after induction, the level of expression exponentially rises. Induction of SOS response system contains more than 40-independent SOS genes that encode proteins engaged in the protection, repair, replication, mutagenesis and DNA metabolism. Under normal growth conditions, SOS genes are expressed at a basic level, as a result of induction, the expression significantly increased. In this study, we designed and analyzed a derivative operator compatible for a broad range of temperatures. In silico analysis of this study showed a very interactive construct that can attract effectively the lexA repressor. This study is a primary modelling study for creation of a new generation of vectors applicable in the industrial biotechnology.

کلیدواژه ها:

SOS ، operator ، interaction ، LexA