The Effect of Berberine on Synaptophysin and Microtubule Associated Protein 2 in An Experimental Model of Alzheimer’s Disease in Male Rat

Background and Objective: Alzheimer s disease is an irreversible neurodegenerative disorder that is caused by age. There is currently no drug that prevents memory loss, cognitive impairmen. the present study aimed to determine the effect of berberine on the amount of synaptophysin and microtubule-associated protein 2 in an experimental model of Alzheimer s disease in rats.Materials and Methods: In this study, male rats weighing 200-250 g were used for 32 heads. Animals were randomly divided into 4 control groups (Sham), Alzheimer s disease group, Alzheimer s model with berberine 10 mg/kg group, and another Alzheimer s model with berberine group receiving 50mg berberine. Male rats were converted to Alzheimer s disease model with help of a stereotactic device, and injectetion Aβ 1-42, 5 μg/microl, in the dorsal hippocampus region. Also, in groups receiving berberine, they received daily from an hour after surgery until one week later. Three weeks after surgery, the mice were made with anesthetized ketamine and homogeneous hippocampal tissue and perforontal area. For this, measurement of synaptophysin and MAP-2 were performed using standard sandwich ELISA. Also, after fixing the left side of the brain in a 4% solution for fixation of paraformaldehyde and preparing a hippocampal block, 5 μm sections were prepared using a microtome system from these sections and stained with H&E staining to determine the density of the neurons in the hippocampal CA1 region.Results: Our Finding in the beta-amyloid group, a significant decrease in synaptophysin and MAP-2 in the pre-frontal and hippocampus was obtained in comparison with the sham group. In addition, the neuronal density of the CA1 region in the amyloid beta group was observed in comparison with the sham group. On the other hand, the treatment of beta-amyloid group with berberine at a dose of 50 mg increased the synaptophysin and MAP-2 in the hippocampus region compared with the amyloid group and prevented the decrease of neuronal CA1.Conclusion: It is concluded that Treatment with berberin in mice with Alzeimer’s disease prevented the reduction of synaptophysin and MAP-2 levels in the hippocampus region and resulted in neuronal protection of the hippocampus.

کلیدواژه ها:

Alzheimer s disease ، Synaptophysin ، Berberine ، Map-2 ، Ca1