Aberrant expression of Igf2 imprinted gene in mouse blastocysts following vitrification at 2-cell stage

Background: Embryo vitrification is an important approach in assisted reproductive techniques (ART) clinics. It helps to prevent ovarian hyperstimulation syndrome and multiple gestations by preserving excess embryos. However, few studies have investigated the effects of vitrification on genomic imprinting of preimplantation embryo.Objective: Thus, this study aimed to assess the effect of vitrification on Igf2 expression as an important imprinted gene in preimplantation embryo.Materials and Methods: Expression of Igf2 in mouse blastocysts from control, IVF and vitrification groups was investigated by qRT-PCR.Results: The results showed that quantitative level of Igf2 gene was significantly lower in blastocysts derived from vitrification in comparison to control and IVF blastocysts (p<0.05).Conclusion: In conclusion, vitrification causes disruption in genomic imprinting mechanism in blastocysts.

کلیدواژه ها:

IVF ، Genomic imprinting ، Igf2 ، Blastocyst