Comparison of two conventional molecular methods in detecting jak2v617f mutations in patients with myeloproliferative neoplasms

Myeloproliferative neoplasms (MPNs) are a heterogeneous group of diseases in which a clonal disorder of hematopoietic stem cells leads to an increase in the level of production in one or more blood cell lines. A link determined more between these groups of diseases through the identification of an acquired mutation tyrosine kinase JAK2. The purpose of this study is two molecular methods as: ARMS-PCR and PCR-RFLP in mutation detection of JAK2V617F.In this study two methods of ARMS-PCR and PCR-RFLP were conducted whit some changes from the previous research study. In order to compare of specificity of these two methods 30 samples were taken from laboratory centers and the reaction of ARMS-PCR and also PCR-RFLP was performed on the samples separately and then the samples were compared whit PCRSEQUENCING.Our results showed us that both ARMSPCR and RFLP-PCR methods have ability to identify and recognize for JAK2V617F, but the method of ARMS-PCR is easier and cheaper than PCR-RFLP. Why the mutation exists in a small proportion of granulocyte population, it is required a sensitive methods for its detection. And in accordance whit the importance of mutation of jak2v617f in chronic myeloid disorders, the discovery of this mutation in the diagnosis and prediction of this response is a useful treatment.

کلیدواژه ها:

MPNs ، JAK2V617F ، ARMS-PCR and PCRRFLP