Study of the beneficial effects of Tsukamurella inchonensisin STZ induced type I diabetic rat intestine

This study was performed to evaluate the probable protective effects of Tsukamurella inchonensis (Ti), as heat-killed Actinomycetales, on the histological and biochemical changes of Wistar rat after experimental diabetes mellitus induced by intraperitoneal injection of 55 mg/kg streptozotocin (STZ). The experiment was carried out by oral administration of Ti at two doses of 105 (low dose) and 107 (high dose) mg/kg body weight in ten rats (in each group) continuously for 14 days. The third group received normal saline as a diabetic control group and the last group mentioned as a negative control (no diabetic, no treatment). The effects of this bacteria were determined on 21st days post study by histopathological examination of the small intestine with a common method. Moreover, biochemical oxidative stress parameters including MDA (Malondialdehyde), SOD (Superoxidase dismutase) and CAT (Catalase) were evaluated on the intestinal tissue. In the present findings, significant differences were observed in the pathological lesions such as degenerative changes in the intestinal mucosa, capillary congestion, submucosal edema and inflammatory cells infiltration which were less severe in both treatment groups, particularly in high dose group. Additionally, MDA, SOD and CAT measurement showed significant differences in both treatment groups compared to diabetic rats. On the basis of the current results, it seems that oral administration of Ti (particularly in high dose) can improve tissue damage induced by diabetes mellitus in Wistar rat. In conclusion, the present results suggest that the heat killed Tsukamurella inchonensiscan have protective effects on diabetes mellitus complications.

کلیدواژه ها:

Tsukamurella inchonensis ، Diabetes mellitus ، Pathological lesions ، Biochemical changes