Synthesis of metal oxide nanocomposites and investigation of the effects on E.coli

Infectious diseases are one of the challenges in the field of medicine. Recently, the use of conventional antibacterial agents has led to microbial resistance to these drugs. This situation is about to reach a dangerous level, which could be the leading cause of mortality in the world in the future. Zinc oxide nanoparticles have anti-cancer, anti-microbial and anti-oxidant activity. This nanoparticle can inhibit the growth of pathogenic bacteria, including Shigella, Escherichia coli and Staphylococcus aureus. Copper oxide nanoparticles are cheap metal oxides. This metal oxide is a p-type semiconductor with optical, catalytic, antifungal, antiviral and antibacterial activity. In this study, the nanocomposite ZnO / CuO was synthesized using [Zn (NO3) 2.6H2O] and [Cu (CH3COO) 2.H2O] by microwave technique. To investigate the antibacterial effects of this material on E. coli, dilution method was performed at concentrations of 0.8-0.05 mg/ml, ZnO, CuO, and ZnO/CuO nanocomposites in Nutrient broth medium with three replications and investigation survival percentage in 600 nm, as well as MIC and NIC determination were used. Structural properties of nanocomposite were investigated using XRD, FT-IR and SEM analyzes. The nanocomposite size of ZnO/CuO was determined using a Debye-Scherer formula of 25.6 nm. Also, the shape of nanomaterials was spherical. Based on the results of bacterial viability, nanocomposite ZnO/CuO at 0.4 mg/ml and with a survival rate of 12% (88% bacterial degradation) concentration has the highest inhibitory effect on E. coli.

کلیدواژه ها:

Metal oxide ، Anti-bacterial ، Nanotechnology ، Copper oxide