Qualitative optimization in DNA Extraction from Date tissue-culture Seedlings by comparing two different methods

The palm propagation is very tedious and expensive because the number of saucers in each tree is limited, so that nowadays tissue culture in palm is commonly used. Tissue culture and micropropagation technique plays a significant role in increasing the production of palm seedlings, however sometimes genetic variation which is called somaclonal variation occurs. The molecular markers are among the biological elements used as laboratory probes to find and identify an individual, tissue, cell, cellnucleus, chromosome or gene and are introduced in various forms of the allele. In order to determine the genetic stability of these plants, we must first get access to their DNA. Therefore, the first step is to determine a DNA extraction method that is fast, safe and cost effective. Various methods have been reported by various researchers to minimize DNA extraction processes. The aim of this study was to optimize DNA extraction from palm seedlings. First, the Doyle& Doyle (1990) method and then the modified Margaritopoulos (2003) method were examined. The quality and quantity of DNA extracted were measured by spectrophotometric and gel electrophoresis methods. Also for extraction of DNA quality, the SSR technique was performed using a random primer called mPdCIR15. Finally, it can be stated that Margaritopoulo (693.85ng.μl-1) method is recommended in case of using RNase, Proteinase K and increasing the washing steps to reduce the amount of contamination as a convenient and cost effective method for DNA extraction in this plant.

کلیدواژه ها:

DNA extraction ، Genetic stability ، Dates