Serological survey of infectious Bluetongue virus disease in ruminant by C-ELISA Test for identification of VP7 in Provinces of IRAN.

Bluetongue disease is an infectious non contaguous viral disease of sheep /goat/cattle and wild ruminants. It is not transmissible to man and is on OIE list disease .The outcome of the infection ranges from inapparent (cattle and goat to fatal with high mortality rate (Sheep). The disease is caused by the bluetongue virus(BTV) /which is member of the orbivirus genus of the family Reoviridae /and transmitted by hematophagic fly of the culicoides. Genus. The clinical signs include febril response characterised by inflammation and congestion facial oedema and haemorragic ulceration of the mucous membranes., in severe cases the tongue may show hyperaemia .swoiien.oedematous and protrude from the mouth. There is severe muscle degenration and mortality up to 15%.The geographic extension in Iran is limited to areas where the vector can survive.This means large zone between the 40th degree north to 35th degree south of Iran which is expending at present almost in all over of provinces as (Fars,Kerman.West&East Azerbiayjan ……….) There are different serogroup( 14) which contain 24 serotypes most of the serogroups appear to be immunological.distinc t/ but there is considerable cross-reaction between memers of the BT serogroups Serological responses usually appear 7-14 days after BTV infection in ruminants and generally long lasting Seroepidemiological methods for diagnosis are AGID (Agar gel immune diffusion test and indirect ELISA are used to detect BT serogroup-specific antibodies although these tests had major drawback of being unable to consistently distinguish between antibodies to viruses in the BT serogroup,.but C- ELISA in the contrary resolve this problem.In this year (1388 (totally 600 sero suspected BT dispatched to the viral diagnosis laboratory and 264 of them were tested .Antibodies in the individual sheep and goat on the competition between the suspected serums and monoclonal antibody which is coupled to the peroxidase This monoclonal antibody is directed to the N-terminal part of the VP7 protein /a major core protein of the BTV(specific for BTV serogroup) The results showed all sero samples are positive which was read at 450 nm long way kbt spectophotometer.As test has proved to be the best for screening antibodies to BTV virus because of the wide pathogenic variability among BTV and the fact is that massive vaccition of ruminant against BTV disease annualy is the only way to controlling it Key

کلیدواژه ها:

BTV-VP7- Orbivirus- Culicoide–Vaccine-Iran