features of black disease and survey of evaluation of pathologic predisposing factors on the artemia

to evaluate pathologic features of black disease This research has been conducted factors on the artemia samples that were supplied from and to survey predisposing artemia. 500 neopilus of artemia were included in this study. urmia research center for in to groups randomly. They were fed till maturity stage. Mature They were divided bearing macroscopic spots were selected and fixated in formalin 10%. They artemia stained with Fontana histochemistry and hematoxilin-Eosin. Histological studies were intracellulary. showed that Black spots in various sizes were present interstitially and were melanin. They Fontana histochemical staining indicated that the black spots out of 500 artemia were 40 .accompanied necrosis, degeneration and reduced tissue cells showed the signs of black disease and (died till maturation. 60 percent of them (n=276 The black spot were observed macroscopically .50 percent of disease (n=138) were died body and especially on thoracic appendage and gills. on antennae under exoskeleton the it was indicated that black spots were present in epithelial ,In microscopic observation tract, muscles and ovary. The observation of external integument of cells of alimentary artemia showed detachment of cuticle from epiderm had occurred. In skeletal few observed. melanin, Zenker necrosis and mild infiltration of leukocytes were muscles isolates vary widely in aeromonas hydrophila is relatively weak pathogens in fish, but of free melanin or lipofusin from pathogen city and cause red sore in fish. The presence culturing of artemia using agricultural by rupture is characteristic. In high density disease is observed when water quality products as food source, the black the composition of the bacterial population. In deteriorates, probably interfering with precipitation dead artemia and crusts of molding of artemia are not culture of artemia if and water is not filtered the microbial load of water rises and results in black evacuated disease. In conclusion to prevent forming of wounds and injuries on external help integument, reduction of stress and displacement of artemia and proper nutrition animals but prevent the disease. Improving these condition dose not save the affected .appears to avoid further losses

کلیدواژه ها:

pathologic-artemia-black disease