Effects of Laser Therapy in The Testicular Tissue of Streptozotocin Induced Diabetic Mice: A Stereological Study

Diabetes mellitus affects the functions of reproductive organs. Spermatogenesis process the evaluation of volume of testis, number of spermatogonia, primary spermatocyte, spermatids, sertoli cells and leydig cells is assessed by carefully observing a stained testis tissue under the microscope. Evaluation of Spermatogenesis process has been considered as one of the most important factors in successful fertilization and determination of testis morphology. In this study, Mice under standard housing conditions were assigned into three experimental groups: (I) control, (II) diabetic , (III) Laser groups (890 nm). The volume of the testis was estimated using the Cavalieri method. The number of spermatogonia, primary spermatocyte, spermatids, sertoli cells and leydig cells was estimated by counting the number of spermatogenic cells in an unbiased counting frame, superimposed on live images of testis tissue using Optical dissector methods. Our results showed a significant difference in the volume of testis and number of spermatogonia, primary spermatocyte, spermatids, sertoli cells and leydig cells in the laser and diabetic groups. This research indicates that the volume of testis and number of spermatogonia, primary spermatocyte, spermatids, sertoli cells and leydig cells is significantly different between diabetic and laser groups. Key word: Stereology, Testis, Diabetic

کلیدواژه ها:

Diabetes Mellitus ، Testise ، Low Level Laser