PCR Detection of Cytomegalovirus in Women With Recurrent Abortions

Background: According to WHO reports, 43% of women and 30.7% of men suffer from infertility. Infectious agents are an important factor in sterility and infertility .Cytomegalovirus is one of the infectious agents that can affect infertility . Rapid diagnosis of this agent can be a pioneer in the treatment and prevention of which moleculartechniques can be used for diagnosis. Purpose and Importance of Research: The purpose of this study was to investigate the role of Cytomegalovirus in infertile women withrecurrent abortions, by PCR method. Methods: Research was conducted on 100 samples of vaginal discharge in women with recurrent abortions from Sarem Hospital inTehran. Samples DNA were extracted by boiling/DNG method and optimized PCR test was performed on samples DNA. The test was also examined in terms of limit of detection (LOD) and specificity. Results: In this study PCR test was optimized and 257bp PCR product was amplified by using of Cytomegalovirus specific primers. Specificity test showed primer 100% specificity.The limit of detection of PCR for CMV was 100 Copy/reaction. 5% of samples were positive by PCR Test.Discussion and Conclusion: This assessment demonstrates the role of Cytomegalovirus in infertility and the occurrence of repeated abortions, which, of course, requires further studies in this context

کلیدواژه ها:

Cytomegalovirus ، Infertility ، Recurrent abortion ، PCR