Association study of rs1874165 & rs2973631 in PRDM9 gene of affected people by azoospermia

Background: Infertility is one of the most common health problems in the world. It affects about 15% of couples, and about half of these cases are a male factor. Male infertility results from multiple genetic and environmental factors that lead to defects in The process of spermatogenesis. Genetic factors include chromosomal abnormalities and single-gene mutations that themselves account for about 10- 15% of factors in male infertility. The PRDM9 gene is present in the region of 5p14.2 and 11 exons. The protein product of this gene is a zinc finger regulatory protein that causes mice to methylate lysine in histone H3. If there is a mutation in the PRDM9, it will stop the Meiosis division.Nucleotide changes in rs1874165 and rs2973631 can interfere with male infertility in some populations. In this study, 100 healthy male fertilized men and 100 azoosperm males were examined for rs1874165 and rs2973631. Methods: After obtaining consent from patients and healthy subjects, blood samples were collected in EDTA containers and then, using the eneAll kit, the gene was extracted and the polymerase chain reaction amplified a specific region of rs1874165 and rs2973631 And thenusing enzyme restriction enzyme MboII for enzymatic digestion for rs1874165 and using the ARMS PCR method for rs2973631, results were finally analyzed using chi-square statistical analysis. Results: The findings of this study indicate that there is a significant difference between rs1874165 and rs2973631 polymorphisms in the 69 PRDM9 gene between healthy and azoospermic groups.