Which Group of Fetal Stem Cells is More Capable of Producing Female Germ Cells

The usage of stem cells in the human new born tissues can be supply a capable source for differentiation of the cells needed. Placenta harbours a plentiful source of various cells with stem cells or stem-like cell properties, which can be used in therapeutic procedures and research. Mesenchymal stem cells (MSCs) have attracted much attention due to their specific differentiation potential and tolerogenic properties. MSCs have been isolated from different parts of placenta; however, in this study, we isolated MSCs from amnion and chorionic membrane, as well as umbilical cord (Wharton’s jelly [WJ]) and compared their capacity regarding differentiation toward female germ cells under influence of 10 ng/mL BMP4. All placenta samples were collected from delivering mothers by normal caesarean section and cells were isolated by different methods. Results showed that all isolated cells were mostly positive for the MSC markers CD73, CD166, and CD105, and minimally reacted with CD34 and CD45 (hematopoietic markers). After differentiation induction using third passage cultured cells, immunocytochemistry staining showed that cells were positive for germ line cell-related genes SSEA4, OCT4, and DDX4, and oocyte-related gene GDF9. QRT-PCR results indicated that human chorionic MSCs (HUMSCs) had a greater potential to be differentiated into female germ linecells. Moreover, the results of this study indicate that human umbilical cord MSCs originated from either male or female umbilical cord have the same differentiation potential into female germ line cells. We recommend that for presumptive application of MSCs for infertility treatment and research, HUMSCs are best candidates due totheir higher differentiation potential, ease of proliferation and expansion, and low immunogenicity.

کلیدواژه ها:

umbilical cord ، germ cells ، mesenchymal stem cells ، differentiation potential ، oocyte ، amniotic membrane