The rate of DNA damage in induced hyperchlostrolemic male rat treated with anethum graveolens seed extract (AGSE)

Background: The aim of the present study was to evaluste the effect of AGSE on the rate of DNA damage in hyperchlostrolemic male rats.Methods: In this experimental study, twenty-four 170±5gr male Wistar rats were allocated to four groups of 6: Control group having a normal diet, hyperghlostromic group (1th group administrated 0.1mg/kg BW sweet almond oil plus cholestrol, 2nd group administrated 0.1mg/kg BW sweet almond oil plus cholesterol plus 500mg/kg extract anethum graveolens seeds), sham group having 500mg/kg BW extract anethum graveolens seeds by oral rout for 48 days. At the end of treatment, rats euthanization,cauda epididymis was used to collect sperm and rate of DNA damage was examined by Acridine orange staining. Data were analyzed with spss and one way ANOVA.Result: The results showed that DNA damage rate decreased significantly (pConclusion: Our results suggested that AGSE may improve DNA damage on hyperchlostromic rat.It can be concluded that AGSE can be considered as a therapeutic strategy to improve high-fat diet effect in DNA damage of male hyperchlostromic rat.

کلیدواژه ها:

DNA damage ، Hyperchlostrolemia ، Anethum graveolen