Long term evaluation of BPS low dose toxicity on in vitro fertilization in female mice

Background: Endocrine disrupting chemicals (EDCs) are the main pollutants in the world that influence human health and reproduction. Bisphenol-A (BPA) is one of the main EDCs found in plastic and cans. Because of endocrine and oxidative stress effect, now a day, companies use BPA substitute in their product. Bisphenol-S, a dominant BPA replacement especially in cans and bottle, is used in BPA-Free product in European countries. However, new investigation revealed dose dependent toxic effect of BPS in many tissue. In our study, BPS low dose toxic effect was investigated on oocyte fertilization and blastocyst rate.Methods: In this study, 40 mature healthy female mice were housed for one week before investigation. All mice were divided to four groups randomly and each group contained ten mice. Mice was administrated normal saline in Control group. Sham group was administrated ethanol (concentration of the ethanol in the media was 0.1%). Three dosages of BPS were administrated 1 μg/kg and 5 μg/kg in different groups. Sub cutaneous (SC) injection was used 21 days for reducing liver first past effect.Result: In all BPS administrated groups, long term BPS absorption induces reduction in oocyte fertility rate and blastocyst formation. Nevertheless, statistical analyses didn t show significant difference between control, sham and BPS absorbed groups. In comparison between BPS administrated groups, 5mg/kg BPS has more destructive effect on oocyte fertilization and blastocyst formation rate, however, the difference between all groups wasn’t meaningful.Conclusion: According to previous studies, BPS as a substitute plays good role in plastic industry because of lesser EDCs effect than BPA. But there is a concern about long term and low dose absorption of Bisphenol family. Our study elucidated low dose consumption of BPS didn’t have prominent effect on In Vitro Fertilization (IVF).

کلیدواژه ها:

Female mice ، In vitro fertilization ، BPS