The relationship between occupational exposure and PPARγ gene expression

Background: Many studies have shown that environmental pollution and chemical materials increased stimulated reactive oxygen species (ROS) production and decline in antioxidant values in human testicles, resulted to decrease in normal morphology, viability and concentration of human sperm and finally disturbed the spermatogenesis. On the other hand, It seems that Peroxisome proliferator-activated receptor gamma (PPARγ) which is one of the most important genes in sperm metabolism, reduces ROS production in human body. The main objective of this survey was to determine and compare PPARγ gene expression in sperm of men with high-risk occupation and men with normal jobs.Methods: Ejaculated sperm have been collected from men (n=80) referred to Royan Institute. The men with exposure in their lives and hard physical jobs were separated from other men. Sperm mRNA was isolated by RNeasy plus universal Mini Kit Qiagen. PPARγ gene expression was determined by real-time PCR. Beta actin was used as house keeping gene. Data were analyzed using the MIXED procedure of SPSS2 program.Result: Our results indicated that PPARγ gene expression in the men with high-risk occupation and exposure are significantly lower than men with normal occupation (p≤0.05).Conclusion: Men with high-risk occupation have expressed significantly lower PPARγ gene compared with men with normal job, and due to the important role of PPARγ gene in human sperm methabolism, it seems that occupation type is so important in men fertility.

کلیدواژه ها:

Gene expression ، High-risk occupation ، Real-time PCR ، ROS ، Exposure ، PPARγ