Detection of Toxoplasma gondii in stray Cats (Felis catus) of Tehran using GRA6 gene

سال انتشار: 1395
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 604

متن کامل این مقاله منتشر نشده است و فقط به صورت چکیده یا چکیده مبسوط در پایگاه موجود می باشد.
توضیح: معمولا کلیه مقالاتی که کمتر از ۵ صفحه باشند در پایگاه سیویلیکا اصل مقاله (فول تکست) محسوب نمی شوند و فقط کاربران عضو بدون کسر اعتبار می توانند فایل آنها را دریافت نمایند.

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

ZOONOSES01_159

تاریخ نمایه سازی: 19 خرداد 1396

چکیده مقاله:

Toxoplasmosis is an endemic disease worldwide and a major public health concern for humans and domestic animals. It is caused by the parasitic protozoan Toxoplasma gondii. Cats and wild felidae play a crucial role in the epidemiology of this disease; they are the only definitive hosts and are therefore the only ones to shed oocysts in their faeces. It is generally assumed that cats play a major role in transmitting T. gondii through the faecal contamination of soil, food or water since they may excrete millions of oocysts over a period of 1–2 weeks. Rather than the consumption of raw or undercooked meat, the ingestion of vegetables or water contaminated with oocysts is currently thought to be the most common route via which humans become infected. It is therefore important to detect cats that are shedding oocysts if control of the disease is to be achieved. In this study the prevalence of Toxoplasma gondii was surveyed by using a nested polymerase chain reaction (nPCR) that was targeted to T. gondii GRA6 gene in stray cats. Biological samples were collected from 50 stray cat that died in accidents or other reasons were collected from different areas of Tehran. The brain of each cat was then removed for direct parasite detection in sterile condition and DNA was extracted with phenol-chloroform method. The 344 bp of GRA6 gene amplified by Nested-PCR method to confirm the presence of Toxoplasma gondii DNA. Results showed, 25/50 cats (50%) were infected with Toxoplasma gondii. This study used brains from stray cats for direct detection since this organ is that most frequently affected. PCR is a sensitive, specific and rapid alternative for detecting T. gondi; Indeed, PCR can detect the DNA of parasites even when the tissues available for testing are in state of decomposition. Because of high occurrence of T. gondii infection in cats in this study, cats may play a serious role in human and other mammalian toxoplasmosis in Tehran.

کلیدواژه ها:

نویسندگان

mahsa esmaeilifallah

ph.d candidate of medical parasitology department of mycology &parasitology School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Javid sadraei

associate professor of department of parasitology faculty of medical science tarbiat modares university tehran ,iran

majid Pirestani

associate professor of department of parasitology faculty of medical science tarbiat modares university tehran ,iran

reza kalantari

ph.d candidate of medical parasitology department of mycology &parasitology School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran