Determination of T-2 Mycotoxin in Fusarium strains by HPLC with fluorescence detector

سال انتشار: 1392
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 616

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تاریخ نمایه سازی: 29 آذر 1395

چکیده مقاله:

T-2 toxin is the most poisonous trichothecene produced by Fusarium species especially F.sporotrichioides. T-2 toxin is a biological contaminant in a number of agriculturalcommodities that can cause severe diseases among humans and animals and even lead to death. The aim of the current study is the analysis of T-2 mycotoxin in Fusarium species by high-performance liquid chromatography (HPLC) combined with florescence detection and derivatization with 1-antroylnitrile (1-AN). Totally,11Fusariumisolates and reference strains were studied. The isolates were tested forthe T-2 toxin production after growing on rice substrate followed by using specific Multisep 225 Trich Clean up columns purification. In this study, T-2 toxin production was ranged from 197.05ug/kg to 8503.07ug/kg. This is the first study of T-2 toxin analysis by HPLC-F in Iran

کلیدواژه ها:


Reza Kachuei

Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Sassan Rezaie

Department of Medical Parasitology and Mycology,Faculty of Public Health, Tehran University of Medial Sciences, Iran

Mohammad Hossein Yadegari

Department of Medical Mycology, Faculty of Medicine Science, Tarbiat Modares University, Tehran, Iran

Naser Safaie

Department of Plant Pathology ,Faculty of Agriculture ,Tarbiat Modares University ,Tehran , Iran