Reza Kachuei - Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Sassan Rezaie - Department of Medical Parasitology and Mycology,Faculty of Public Health, Tehran University of Medial Sciences, Iran
Mohammad Hossein Yadegari - Department of Medical Mycology, Faculty of Medicine Science, Tarbiat Modares University, Tehran, Iran
Naser Safaie - Department of Plant Pathology ,Faculty of Agriculture ,Tarbiat Modares University ,Tehran , Iran

T-2 toxin is the most poisonous trichothecene produced by Fusarium species especially F.sporotrichioides. T-2 toxin is a biological contaminant in a number of agriculturalcommodities that can cause severe diseases among humans and animals and even lead to death. The aim of the current study is the analysis of T-2 mycotoxin in Fusarium species by high-performance liquid chromatography (HPLC) combined with florescence detection and derivatization with 1-antroylnitrile (1-AN). Totally,11Fusariumisolates and reference strains were studied. The isolates were tested forthe T-2 toxin production after growing on rice substrate followed by using specific Multisep 225 Trich Clean up columns purification. In this study, T-2 toxin production was ranged from 197.05ug/kg to 8503.07ug/kg. This is the first study of T-2 toxin analysis by HPLC-F in Iran

Fusarium, Trichothecene, T-2 toxin, HPLC-F