Improvement of maize (Zea mays L.) anther culture embryogenesis and direct regeneration by differentplant growth regulators and gelling agent
سال انتشار: 1392
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 666
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شناسه ملی سند علمی:
JR_JABR-1-1_003
تاریخ نمایه سازی: 29 آذر 1395
چکیده مقاله:
Androgenesis via anther culture or microspore culture is one of the current methods for producing haploid and double haploid plants in maize. To use of this method in maize breeding program it should be able to regenerate enough plantlets. Anthers culture usually is carried out indirectly via callus induction and regeneration on atleast two different media. In this study a responsive genotype, ETH-M82, using a new single culture medium was used for embryogenesis and regeneration. We tested different growth regulators (2,4-D; Kinetin; NAA & IAA) in modified YP medium.After6-week, direct regeneration on some treatments was observed. The highest frequency of direct formation of plantlets (in 100 anthers) occurred on medium supplemented with 2mgl-1 IAA and 2mgl-1 kinetin (4%).Best results with an average of 3.1plantlets in replication were obtained with the medium solidified with agar,while in difcobactoagar only 1.4 of plantlets in replication was produced. This experiment suggested that agar and plant growth regulators in the medium were beneficial for producing embryo and plantlet from maize anthers
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نویسندگان
Seyedeh Zahra Hosseini
Department of Plant Biology, Faculty of Basic Sciences, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran
Ahmad Ismaili
Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, KhoramAbad, Iran
Payam Pour Mohammadi
Department ofBiotechnology and Plant Breeding, Faculty of Agriculture, Ramin University of Agriculture and Natural Resources, Khouzestan, Iran