Isolation and molecular identification of mycoplasma genitalium from the secretion of genital tract in infertile male and female
سال انتشار: 1393
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 516
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شناسه ملی سند علمی:
JR_IJRM-12-9_002
تاریخ نمایه سازی: 16 شهریور 1395
چکیده مقاله:
Background: Mycoplasmas can cause acute and chronic diseases at multiple sites with wide-range complications and have been implicated as cofactors in diseases. The infections influenced form genital mycoplasmas specifically Mycoplasma hominis and Mycoplasma genitalium potentially affect reproductive disorders, and infertility.Objective: Isolation and molecular identification of Mycoplasma genitalium from the genital tract of infertile male and vaginal discharge of infertile female referred to Infertility Center of Kerman in 2013.Materials and Methods: This study was a randomized, prospective study. We included 100 infertile male and 100 infertile female that were referred to the Infertility Center of Kerman. Then for isolation and molecular identification of Mycoplasma genitalium from urethral and vaginal discharge polymerase chain reaction was performed on Mycoplasma genus and genitalium.Results: From a total of 100 semen samples 45 patients (45%) were mycoplasma-positive and 13 (28.8%) were genitalium species positive. Also, from a total of 100 women samples 43 women (43%) were mycoplasma-positive and 10 (23.2%) were genitalium species positive. Positive samples were sequenced and phylogenetic tree was drawn.Conclusion: According to the results of this study, a high percentage of infertile male and female were infected with the Mycoplasma genitalium. For prevention of harmful and significant consequences of this infection, we suggest a screening program in symptomatic infertile couples.
کلیدواژه ها:
نویسندگان
Naeime Mohseni Moghadam
Department of IVF, Afzalipoor Hospital, IVF Center, Kerman, Iran
Babak Kheirkhah
Department of Microbiology, College of Science, Kerman Branch, Islamic Azad University, Kerman, Iran
Toraj Reza Mirshekari
Department of Pathology, Afzalipoor Medical College, Kerman, Iran.
Majid Fasihi Harandi
Department of Parasitology, Afzalipoor Medical College, Kerman, Iran