Diagnosis of brucellosis by use of BACTEC blood culture and confirmation by PCR

Brucellosis is one of the most common zoonotic diseases in Iran. Growth of Brucella is slowand blood cultures of these bacteria are time-consuming via classical methods. We try toevaluate BACTEC 9120 system capacity in order to detect of bacteremia due to Brucella spp andto confirm isolated bacteria by PCR. Blood culture sample of 102 suspected patients evaluatedby BACTEC 9120 system. They were subcultured when the machine detected their growth; ifnot; blind subcultures were performed on days 7, 14, 21 and 28. Forty-one of 102 suspectedpatients showed bactermia. Isolation rate of Brucella was 40.2%. All patients were detected byBACTEC 9120 system. All positive blood culture was detected via BACTEC 9120 and blindsubcultures. No positive blood culture bottles were missed by the system. Our data obtained byusing the BACTEC 9120 system indicates a more rapid detection of Brucella than conventionalmethods.

کلیدواژه ها:

brucellosis ، BACTEC system ، blood culture