Evaluation of phytochemical, and antifungal effect of Syzygium aromaticum Essential oil

Fungal infections have increased over the last two decades, and Candida spp. are the most common cause of fungal infections in humans. C. albicans, is usually associated with pathological conditions. These species have the potential to develop antifungal resistance either intrinsically or during treatment. Nowadays, there is a great demand for novel antifungals, and utilization of natural compounds such as plant Essential oils instead of antifungal drugs against fungal pathogens has enhanced. In this study, we aimed to evaluate the antifungal activity of Syzygium aromaticum Essential oil against Candida spp. by using broth microdillution method to determine minimum inhibitory concentrations and minimal fungicidal concentrations. The Syzygium aromaticum Essential oil was extracted by hydrodistillation technique using Clevenger apparatus. The chemical compositions of EO were analyzed by an optimized gas chromatography mass spectrometry (GC–MS) method. Analysis of the EO showed chemical profile dominated by Eugenol (۷۷.۶۳%). The obtained results of MIC for Syzygium aromaticum EO was between ۰.۱۵-۰.۳۱۲ µl/ml and ۰.۳۱۲-۱.۲۵ µl/ml for MFC. With attention to resistance of different Candida spp to synthetic and chemical antifungals, herbal compositions such as Syzygium aromaticum Essential oil are safe replacement treatments for cutaneous and mucosal candidia infections.

کلیدواژه ها:

phytochemical ، antifungal ، Syzygium aromaticum ، Essential oil