Organoids and ۳D Cell Culture: Opportunities and Future Directions in Medical Research

An organoid is a collection of organ-specific cell types that develops from stem cells or organ progenitors and self-organises through cell sorting and spatially restricted lineage commitment, like in vivo. Three-dimensional (۳D) in vitro cell culture provides a window to human disease and development by creating tissue structures that mimic the architecture and functionality of tissues in vivo. This has revolutionized biomedical research, providing a more advanced platform for diagnostics, drug discovery, and personalized therapeutics. Culturing cells in ۳D typically takes longer, is less reproducible, and is more complex to characterize than ۲D cultures. Researchers are increasingly looking to ۳D cell models to bridge the translational gap between ۲D cell cultures and in vivo conditions. These cell models more closely represent the microenvironments, cell-to-cell interactions, and biological processes that occur in vivo. The last decade has seen huge development in ۳D culture technology, from identifying essential factors in the culture environment for controlling growth and development, to the development of technologies to help automate and streamline the culture process. However, ۳D cell culture comes with its own unique set of challenges, and ensuring the validity and reliability of your experimental results requires careful planning, optimization, and sometimes a lot of patience. This paper will address some of the common pitfalls in ۳D culture and provide tips and tricks to help optimize ۳D culture workflow, from cell selection to culture maintenance and post-culture characterization.

کلیدواژه ها:

۳D Cell Culture ، Organoids ، ECM ، ESCs ، IPSCs