Spatial distribution changes of cells in central nervous system after peripheral nerve injury

The spatial distribution of the cells is strongly associated with their interactions and cellular behavior isDetermined by spatial cellular distribution. The cell-to-cell interaction relates to normal functioning of the organand changes in these cell -to-cell interactions can result in abnormal tissue physiology related to disease. Thisstudy examined the ۳D spatial arrangements of the neurons and glial cells within rat spinal cord after Sciaticaxotomy. Sixteen adult male rats were divided to control and axotomy groups. After ۱۲ weeks, spinl cords wereremoved for first- and second-order stereological Assessments. Second-order stereological studies were appliedto evaluate the pair correlation and cross-correlation functions using a dipole probe superimposed onto thespinal cord sections. The findings revealed ۷% and ۳۶% reductions in the mean volume and total number ofmotoneurons, respectively, and a ۲۵% increase in the neuroglial cell number in the axotomized rats comparedto the control rats. In contrast, the anterior horn volume remained unchanged. The results also indicated abroader gap in the pair correlation curve for the motoneurons and neuroglial cells in the axotomized ratscompared to the control rats. _is finding shows a negative correlation for the distribution of motoneurons andneuroglial cells in the axotomized rats. The cross-correlation curve shows a negative correlation between themotoneurons and neuroglial cells in the axotomized rats. These findings suggest that cellular structural andfunctional changes after sciatic nerve injury lead to the alterations in the spatial arrangement of motoneuronsand neuroglial cells, finally affecting the normal function of the central nervous system.