Effects of curcumin, vitamin D and vitamin E with Fe۲O۳-NPs in scrotal hyperthermia

Background: Infertility is one of the major factors affecting most people around the world. short term exposure to high temperatures can cause hyperthermia, which is one of the causes of male infertility. The aim of this study was to investigate the protective effect of curcumin, vitamins D and E along with Iron (III) oxide nanoparticles (Fe۲O۳-NPs) on semen parameters and its effect on Bax and Bcl-۲ expression.Material and Methods: In this study, the lower part of the rat was exposed to ۴۳ ° C for ۵ weeks every other day for ۵ weeks. Then the animals were killed. Tissue samples were taken to analyze sperm and assess the level of apoptosis in germ cells and RNA extraction to evaluate the expression of Bax and Bcl-۲ genes.Results: The results of this study showed that administration of curcumin, vitamin D and vitamin E with Fe۲O۳-NPs can improve the parameters of semen, Bax geneexpression and Bcl-۲ as well as in rats with testicular hyperthermia. In addition,curcumin by reducing the toxicity of Fe۲O۳ nanoparticles was able to reduce itsnegative effects and also reduce apoptosis in germ cells. This decrease in apoptosis was attributed to decreased Bcl-۲ gene expression and increased expression of Bax.Conclusion: All the results of this study confirmed that Fe۲O۳-NPs containing antioxidants or vitamins are useful in improving fertility in rats due to scrotal hyperthermia. Although Fe۲O۳-NPs containing both antioxidants and vitamins had a greater effect on improving fertility and reducing the toxic effects of nanoparticles.

کلیدواژه ها:

Nanoparticles ، Apoptosis ، Curcumin ، Vitamin D ، Vitamin E.