Chromosome number report and karyotype analysis of S. litwinowii (Caryophyllaceae)

سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 95

نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

BIOCONF21_0906

تاریخ نمایه سازی: 7 شهریور 1400

چکیده مقاله:

The genus Silene including about ۲۴ species in Khorasan (NE Iran) of which ۳ species are endemic of this province. Silene litwinowii is an interesting species with very restricted distribution in Khorasn province. There is no information about chromosome number and karyotype features of the species. Therefore, the aim of this research is karyotype analysis of S. liwinowii. The Maturated seeds was collected from natural habitat of the species in Khorasan province. For karyotype study, freshly grown roots tips was pretreated for ۳ hours in ۸-hydroxy quinolone and then fixed in carnoy solution for ۲۴ hours. After hydrolysis, staining with aceto-orsein and Squash, the well-prepared metaphase plates was photographed by digital camera. Karyotype features of this species including chromosomal formula, karyotype symmetry, A۱, A۲, TF% coefficients and the largest to smallest chromosome ratio were calculated. As a results the chromosome number of the species was recognized to be ۲n=۲x=۲۴ and reported for the first time. The Stebbins's asymmetry class, A۱ asymmetry index, TF% index and L/S were determined to be ۲A, ۰.۹۴, ۴۰.۹۱ and ۲.۸۱ respectively. The karyotype formula of this species consists of ۱۰ submetacentric (m) and ۲ Metacentric (M) chromosomes. According to the results of the present study, this species has a relatively symmetrical karyotype.

نویسندگان

Abbas Gholipour

Associated professor, Payame Noor University, Tehran, Iran

Fatemeh Abbaszade

MSc graduated of plant development biology, Payame Noor University, Tehran, Iran

Dawood Zakeri

MSc of desertification combating, Isfahan University of Technology, Isfahan, Iran